LOCUS       sequence-114824-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid expresses full-length human TET3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Ko M, An J, Bandukwala HS, Chavez L, Aijo T, Pastor WA, Segal MF, Li
            H, Koh KP, Lahdesmaki H, Hogan PG, Aravind L, Rao A
  TITLE     Modulation of TET2 expression and 5-methylcytosine oxidation by the 
            CXXC domain protein IDAX.
  JOURNAL   Nature. 2013 May 2;497(7447):122-6. doi: 10.1038/nature12052. Epub 
            2013 Apr 7.
  PUBMED    23563267
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12052"; journalName: "Nature"; date: "2013-05-2- 2"; 
            volume: "497"; issue: "7447"; pages: "122-6"
COMMENT     Sequence Label: FH-TET3-pEF
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..32
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13..31
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      64..73
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             73..96
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             109..135
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             109..135
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ttagcttggt actaatacga ctcactatag ggagacccaa gctggctagg taagcttggt
       61 accgccacca tggactacaa ggacgacgat gacaagctcg atggaggata cccctacgac
      121 gtgcccgact acgccggagg actcgacagc cagtttcagg tgcccctggc cgtccagccg
      181 gacctgccag gcctttatga cttccctcag cgccaggtga tggtagggag cttcccgggg
      241 tctgggctct ccatggctgg gagtgagtcc caactccgag ggggtggaga tggtcgaaag
      301 aaacggaaac ggtgtggtac ttgtgagccc tgccggcggc tggaaaactg tggcgcttgc
      361 actagctgta ccaaccgccg cacgcaccag atctgcaaac tgcgaaaatg tgaggtgctg
      421 aagaaaaaag tagggcttct caaggaggtg gaaataaagg ctggtgaagg agccgggccg
      481 tggggacaag gagcggctgt caagacaggc tcagagctca gcccagttga tggacctgtt
      541 ccaggtcaga tggactcagg gccagtgtac catggggact cacggcagct aagcgcctca
      601 ggggtgccgg tcaatggtgc tagagagccc gctggaccca gtctgctggg gactgggggt
      661 ccttggcggg tagaccaaaa gcccgactgg gaggctgccc caggcccagc tcatactgct
      721 cgcctggaag atgcccacga tctggtggcc ttttcggctg tggccgaagc tgtgtcctct
      781 tatggggccc ttagcacccg gctctatgaa acctt
//
