LOCUS       sequence-114839-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synchronize trafficking of EGFP-GPI from the ER (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: Ii-Str_SBP-EGFP-GPI
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     152..211
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             212..322
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     primer_bind     complement(374..395)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(635..654)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
       61 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
      121 gataagcttg ccacaacccg ggaggcgcgc catgtacagg atgcaactcc tgtcttgcat
      181 tgcactaagt cttgcacttg tcacgaattc cgacgagaag accactggtt ggcgaggtgg
      241 acacgttgtt gaaggactgg ctggggaact tgaacaactt cgtgcacgac tggagcatca
      301 cccacaaggt caacgtgaac cacctgcagg tgtgagcaag ggcgaggagc tgttcaccgg
      361 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      421 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      481 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
      541 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      601 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      661 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      721 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca g
//
