LOCUS       sequence-114842-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ManII reporter only (no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: ManII-SBP-EGFP_puromycin
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             510..623
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     primer_bind     complement(678..699)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc gagctcggat cgatatctgc ggcctagcta
      121 gcgggaggcg cgccatgaag ttaagtcgcc agttcaccgt gtttggcagc gcgatcttct
      181 gcgtcgtaat cttctcactc tacctgatgc tggacagggg tcacttggac taccctcggg
      241 gcccgcgcca ggagggctcc tttccgcagg gccagctttc aatattgcaa gaaaagattg
      301 accatttgga gcgtttgctc gctgagaaca acgagattat ctcaaatatc agagactcag
      361 tcatcaacct gagcgagtct gtggaggacg gcccgcgggg gtcaccaggc aacgccagcc
      421 aaggctccat ccacctccac tcgccacagt tggccctgca ggctgacccc agagactgtt
      481 tggatcccac cggtcgccac cggaattcca tggacgagaa gaccactggt tggcgaggtg
      541 gacacgttgt tgaaggactg gctggggaac ttgaacaact tcgtgcacga ctggagcatc
      601 acccacaagg tcaacgtgaa ccacctgcag gtatggtgag caaaggcgag gagctgttca
      661 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      721 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      781 ccaccggcaa gctgcccgtg ccct
//
