LOCUS       sequence-114843-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ManII reporter only (no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: ManII-SBP-EGFP_puromycin
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..124)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     primer_bind     complement(562..581)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(563..581)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(626..677)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(655..675)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tacctgcagg tggttcacgt tgaccttgtg ggtgatgctc cagtcgtgca cgaagttgtt
       61 caagttcccc agccagtcct tcaacaacgt gtccacctcg ccaaccagtg gtcttctcgt
      121 ccatggaatt ccggtggcga ccggtgggat ccaaacagtc tctggggtca gcctgcaggg
      181 ccaactgtgg cgagtggagg tggatggagc cttggctggc gttgcctggt gacccccgcg
      241 ggccgtcctc cacagactcg ctcaggttga tgactgagtc tctgatattt gagataatct
      301 cgttgttctc agcgagcaaa cgctccaaat ggtcaatctt ttcttgcaat attgaaagct
      361 ggccctgcgg aaaggagccc tcctggcgcg ggccccgagg gtagtccaag tgacccctgt
      421 ccagcatcag gtagagtgag aagattacga cgcagaagat cgcgctgcca aacacggtga
      481 actggcgact taacttcatg gcgcgcctcc cgctagctag gccgcagata tcgatccgag
      541 ctcggtacca agcttgggtc tccctatagt gagtcgtatt aatttcgata agccagtaag
      601 cagtgggttc tctagttagc cagagagctc tgcttatata gacctcccac cgtacacgcc
      661 taccgcccat ttgcgtcaat
//
