LOCUS       sequence-114845-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  VSVG reporter only (no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: VSVGwt-SBP-EGFP_puromycin
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..44
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        25..43
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttggta
       61 ccgagctcgg atcgatatct gcggcctagc tagcgggagg cgcgccatga agtgcctttt
      121 gtacttagcc tttttattca ttggggtgaa ttgcaagttc accatagttt ttccacacaa
      181 ccaaaaagga aactggaaaa atgttccttc taattaccat tattgcccgt caagctcaga
      241 tttaaattgg cataatgact taataggcac agccttacaa gtcaaaatgc ccaagagtca
      301 caaggctatt caagcagacg gttggatgtg tcatgcttcc aaatgggtca ctacttgtga
      361 tttccgctgg tatggaccga agtatataac acattccatc cgatccttca ctccatctgt
      421 agaacaatgc aaggaaagca ttgaacaaac gaaacaagga acttggctga atccaggctt
      481 ccctcctcaa agttgtggat atgcaactgt gacggatgcc gaagcagtga ttgtccaggt
      541 gactcctcac catgtgctgg ttgatgaata cacaggagaa tgggttgatt cacagttcat
      601 caacggaaaa tgcagcaatt acatatgccc cactgtccat aactctacaa cctggcattc
      661 tgactataag gtcaaagggc tatgtgattc taacctcatt tccatggaca tcaccttctt
      721 ctcagaggac ggagagctat cat
//
