LOCUS       sequence-114847-         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  VSVG reporter only (no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: VSVGwt-SBP-EGFP_puromycin
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..125)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             complement(135..167)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 atacctgcag gtggttcacg ttgaccttgt gggtgatgct ccagtcgtgc acgaagttgt
       61 tcaagttccc cagccagtcc ttcaacaacg tgtccacctc gccaaccagt ggtcttctcg
      121 tccatggaat tcagctttcc aagtcggttc atctctatgt ctgtataaat ctgtcttttc
      181 ttggtgtgct ttaatttaat gcaaagatgg ataccaactc ggagaaccaa gaatagtcca
      241 atgattaacc ctatgataaa gaaaaaagag gcaatagagc ttttccaact actgaaccaa
      301 ccttctacaa gctcgattgg atttttggat agcccagtat caccaaaaaa taaactctca
      361 tcatcaggaa gttgcgaagc agcgtcttga atgtgaggat gttcgaacac ctgagccttt
      421 gagctaagat gaagatcgga gtccaacata ccatgtccaa tcatgtataa aggaaactta
      481 tatcctgaac tggtcctcag aactccattg ggtccaattt ccacgtcttc atatggtgcc
      541 cagtcatccc acagttccct ttctgtggta gttccactga tcattccgac cattcttgag
      601 aggattggag cagcaatatc gactctgatg tatctggtct caaagtattt tagggtacca
      661 ttgattatgg t
//
