LOCUS       sequence-114858-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RCAN1 aa1-150 expression with CMV promoter.  Flag tagged..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Liu Q, Busby JC, Molkentin JD
  TITLE     Interaction between TAK1-TAB1-TAB2 and RCAN1-calcineurin defines a 
            signalling nodal control point.
  JOURNAL   Nat Cell Biol. 2009 Feb;11(2):154-61. doi: 10.1038/ncb1823. Epub 
            2009 Jan 11.
  PUBMED    19136967
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1823"; 
            journalName: "Nat Cell Biol"; date: "2009-02"; volume: "11"; issue: 
            "2"; pages: "154-61"
COMMENT     Sequence Label: RCAN1-aa1-150
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..80
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      559..568
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    572..706
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(609..628)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     663..682
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        775..966
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(798..818)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ttctctccac aggtgtccac tcccaggtcc aactgcacct cggttctatc gatatggact
       61 acaaagacga tgacgacaag cgaattcccc ggggatccat ggaggaggtg gatctgcagg
      121 acctgccgag cgccaccatc gcctgccacc tggacccgcg cgtgttcgtg gacggcctgt
      181 gccgggccaa atttgaatcc ctcttcagaa catatgacaa ggacaccacc ttccagtatt
      241 ttaagagctt caaacgtgtc cggataaact tcagcaaccc cttatctgca gccgatgcca
      301 ggctgcggct gcacaagacc gagttcctgg ggaaggaaat gaagttgtat tttgctcaga
      361 ctttacacat aggaagttca cacctggctc cgcccaatcc cgacaaacag ttcctcatct
      421 cccctccggc ctctcctccc gttggctgga aacaagtaga agatgccacc cccgtcataa
      481 attacgatct tttatatgcc atctccaagc tggggccagg agagaagtat gaactgcatg
      541 cagcgacatg aaagcttggc cgccatggcc caacttgttt attgcagctt ataatggtta
      601 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
      661 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcggga attaattcgg
      721 cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg
      781 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
      841 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
      901 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
      961 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     1021 gccccatggc tgactaattt ttt
//
