LOCUS       sequence-114859-        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD L459G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS526 (TJF093)
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..50)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    159..183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(183..202)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(184..202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(291..308)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt accagactcg
       61 agggtaccga cgtcagcgat cgcgtggccg gccgatatcc aattgagatc tgccatatgt
      121 atatctcctt cttatactta actaatatac taagatgggg aattgttatc cgctcacaat
      181 tcccctatag tgagtcgtat taatttcgat tatgcggccg tgtacaatac gattactttc
      241 tgttcgactt aagcattatg cggccgcaag cttggatcct cattcattca gtgatgatga
      301 tggtgatgag aagaacctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat
      361 tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc
      421 ttcggtactt tgtagcccgt gagctgacgg cggcaaaagg tcaccagtga ctcttcggta
      481 agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt
      541 acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg
      601 ttgggataga cgttaaaacc ggaaacgcca atcatgtctt ttttacgatc gacaatgcgc
      661 aggaaccctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg
      721 atctcatctg tagcatccgg acgctgccag taacccagca tcacctgcgg tcctttgaca
      781 caaagctcac ccggttgacc cggtggtact tcattatcat catcatccac cagtttggct
      841 tccgtcgacg gcaccggcaa accgatgcta ccactatgat aatcaatatc atatgggtta
      901 acgctgacca gcggcgcaca ctcggtaagg ccatagcctt ccagcagata ctgtcctgtc
      961 agtttcaccc aacgctctgc caccacttgc tgcactggca tccctccgcc tgcggaaaga
     1021 tgcagactgg agaaatccag ctgctggaac tccttattgt tcagcacgca
//
