LOCUS       sequence-114864-        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RCAN1 aa1-150 expression with CMV promoter.  Flag tagged..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Liu Q, Busby JC, Molkentin JD
  TITLE     Interaction between TAK1-TAB1-TAB2 and RCAN1-calcineurin defines a 
            signalling nodal control point.
  JOURNAL   Nat Cell Biol. 2009 Feb;11(2):154-61. doi: 10.1038/ncb1823. Epub 
            2009 Jan 11.
  PUBMED    19136967
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1823"; 
            journalName: "Nat Cell Biol"; date: "2009-02"; volume: "11"; issue: 
            "2"; pages: "154-61"
COMMENT     Sequence Label: RCAN1-aa1-150
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    81..215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(105..124)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     159..178
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     regulatory      219..228
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(707..730)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(773..792)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(805..823)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(806..823)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1033..1057)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tttccgcctc agaaggtacc taaccaagtt cctctttcag aggttatttc aggccatggt
       61 gctgcgccga attaattccc gatccagaca tgataagata cattgatgag tttggacaaa
      121 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
      181 tatttgtaac cattataagc tgcaataaac aagttgggcc atggcggcca agctttcatg
      241 tcgctgcatg cagttcatac ttctctcctg gccccagctt ggagatggca tataaaagat
      301 cgtaatttat gacgggggtg gcatcttcta cttgtttcca gccaacggga ggagaggccg
      361 gaggggagat gaggaactgt ttgtcgggat tgggcggagc caggtgtgaa cttcctatgt
      421 gtaaagtctg agcaaaatac aacttcattt ccttccccag gaactcggtc ttgtgcagcc
      481 gcagcctggc atcggctgca gataaggggt tgctgaagtt tatccggaca cgtttgaagc
      541 tcttaaaata ctggaaggtg gtgtccttgt catatgttct gaagagggat tcaaatttgg
      601 cccggcacag gccgtccacg aacacgcgcg ggtccaggtg gcaggcgatg gtggcgctcg
      661 gcaggtcctg cagatccacc tcctccatgg atccccgggg aattcgcttg tcgtcatcgt
      721 ctttgtagtc catatcgata gaaccgaggt gcagttggac ctgggagtgg acacctgtgg
      781 agagaaaggc aaagtggatg ttattctata gtgtcaccta aatcgtatgt gtatgataca
      841 taaggttatg tattaattgt agccgcgttc taacgaagcc aagggggtgg gcctatagac
      901 tctataggcg gtacttacgt cactcttggc acggggaatc cgcgttccaa tgcaccgttc
      961 ccggccgcgg aggctggatc ggtcccggtg tcttctatgg aggtcaaaac agcgtggatg
     1021 gcgtctccag gcgatctgac ggttcactaa acgagctctg cttatat
//
