LOCUS       sequence-114865-        6322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An amino-terminal YFP fragment was fused to Gbeta-5. When 
            co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a
            Ggamma subunit, a fluorescent signal is produced..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Hynes TR, Tang L, Mervine SM, Sabo JL, Yost EA, Devreotes PN, Berlot
            CH
  TITLE     Visualization of G protein betagamma dimers using bimolecular 
            fluorescence complementation demonstrates roles for both beta and 
            gamma in subcellular targeting.
  JOURNAL   J Biol Chem. 2004 Jul 16;279(29):30279-86. Epub 2004 May 10.
  PUBMED    15136579
REFERENCE   2  (bases 1 to 6322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-07-16"; volume: "279(29)"; pages: "30279-86. 
            Epub 2004 May 10"
COMMENT     Sequence Label: YFP(1-158)-beta-5 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..6322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     471..490
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(603..1061)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(740..761)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     955..974
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(1186..1204)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1272..1376
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1377..1445,1446..2237)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1595..1614)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     enhancer        2310..2689
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2690..2893
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2844..2864
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2938..2957
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2938..2956
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2988..2997
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2994..2996,2997..2999,3000..3458)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(3039..3060)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3300..3319)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(4594..4612)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4595..4612)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          4696..4761
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4891..4911
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5183..5317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5220..5239)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5274..5293
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6163..6298
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
       61 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      121 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      181 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      241 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      301 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      361 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      421 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      481 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      541 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct
      601 agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
      661 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
      721 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      781 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc
      841 acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      901 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      961 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     1021 caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
     1081 gaccgtataa cgtgctttcc tcgttggaat cagagcggga gctaaacagg aggccgatta
     1141 aagggatttt agacaggaac ggtacgccag ctggattacc aaagggcctc gtgatacgcc
     1201 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     1261 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1321 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     1381 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     1441 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     1501 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     1561 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     1621 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     1681 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1741 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1801 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1861 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1921 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1981 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     2041 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     2101 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     2161 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     2221 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     2281 aacttcattt ttaatttctc tagcgcgttg acattgatta ttgactagtt attaatagta
     2341 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2401 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2461 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggactattt
     2521 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2581 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2641 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2701 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2761 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2821 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2881 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
     2941 tacgactcac tatagggaga cccaagcttg gtaccgagct cggatccgcc accatggtga
     3001 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3061 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     3121 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     3181 ccaccttcgg ctacggcctg atgtgcttcg cccgctaccc cgaccacatg aagcagcacg
     3241 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     3301 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     3361 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     3421 agtacaacta caacagccac aacgtctata tcatggccga caagcagaga tccatcgcaa
     3481 ccatggcaac cgaggggctg cacgagaacg agacgctggc gtcgctgaag agcgaggccg
     3541 agagcctcaa gggcaagctg gaggaggagc gagccaagct gcacgatgtg gagctgcacc
     3601 aggtggcgga gcgggtggag gccctggggc agtttgtcat gaagaccaga aggaccctca
     3661 aaggccacgg gaacaaagtc ctgtgcatgg actggtgcaa agataagagg aggatcgtga
     3721 gctcgtcaca ggatgggaag gtgatcgtgt gggattcctt caccacaaac aaggagcacg
     3781 cggtcaccat gccctgcacg tgggtgatgg catgtgctta tgccccatcg ggatgtgcca
     3841 ttgcttgtgg tggtttggat aataagtgtt ctgtgtaccc cttgacgttt gacaaaaatg
     3901 aaaacatggc tgccaaaaag aagtctgttg ctatgcacac caactacctg tcggcctgca
     3961 gcttcaccaa ctctgacatg cagatcctga cagcgagcgg cgatggcaca tgtgccctgt
     4021 gggacgtgga gagcgggcag ctgctgcaga gcttccacgg acatggggct gacgtcctct
     4081 gcttggacct ggccccctca gaaactggaa acaccttcgt gtctggggga tgtgacaaga
     4141 aagccatggt gtgggacatg cgctccggcc agtgcgtgca ggcctttgaa acacatgaat
     4201 ctgacatcaa cagtgtccgg tactacccca gtggagatgc ctttgcttca gggtcagatg
     4261 acgctacgtg tcgcctctat gacctgcggg cagataggga ggttgccatc tattccaaag
     4321 aaagcatcat atttggagca tccagcgtgg acttctccct cagtggtcgc ctgctgtttg
     4381 ctggatacaa tgattacact atcaacgtct gggatgttct caaagggtcc cgggtctcca
     4441 tcctgtttgg acatgaaaac cgcgttagca ctctacgagt ttcccccgat gggactgctt
     4501 tctgctctgg atcatgggat cataccctca gagtctgggc ctaaagatct atcacactgg
     4561 cggccgctcg agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagg
     4621 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     4681 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     4741 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     4801 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     4861 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     4921 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     4981 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     5041 atggaaaaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     5101 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     5161 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     5221 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5281 gtccaaactc atcaatgtat cttatcatgt ctggatcatc ccgccatggt atcaacgcca
     5341 tatttctatt tacagtaggg acctcttcgt tgtgtaggta ccgctgtatt cctagggaaa
     5401 tagtagaggc accttgaact gtctgcatca gccatatagc ccccgctgtt cgacttacaa
     5461 acacaggcac agtactgaca aacccataca cctcctctga aatacccata gttgctaggg
     5521 ctgtctccga actcattaca ccctccaaag tcagagctgt aatttcgcca tcaagggcag
     5581 cgagggcttc tccagataaa atagcttctg ccgagagtcc cgtaagggta gacacttcag
     5641 ctaatccctc gatgaggtct actagaatag tcagtgcggc tcccattttg aaaattcact
     5701 tacttgatca gcttcagaag atggcggagg gcctccaaca cagtaatttt cctcccgact
     5761 cttaaaatag aaaatgtcaa gtcagttaag caggaagtgg actaactgac gcagctggcc
     5821 gtgcgacatc ctcttttaat tagttgctag gcaacgccct ccagagggcg tgtggttttg
     5881 caagaggaag caaaagcctc tccacccagg cctagaatgt ttccacccaa tcattactat
     5941 gacaacagct gtttttttta gtattaagca gaggccgggg acccctgggc ccgcttactc
     6001 tggagaaaaa gaagagaggc attgtagagg cttccagagg caacttgtca aaacaggact
     6061 gcttctattt ctgtcacact gtctggccct gtcacaaggt ccagcacctc cataccccct
     6121 ttaataagca gtttgggaac gggtgcgggt cttactccgc ccatcccgcc cctaactccg
     6181 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6241 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6301 ggcttttgca aaaagctaat tc
//
