LOCUS       sequence-114868-        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD R554G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS518 (TJF085)
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    160..184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(184..203)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(185..203)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(292..309)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagacgagtc catggtggct ggcgttcaaa tttcgcagca gcggtttctt taccagactc
       61 gagggtaccg acgtcagcga tcgcgtggcc ggccgatatc caattgagat ctgccatatg
      121 tatatctcct tcttatactt aactaatata ctaagatggg gaattgttat ccgctcacaa
      181 ttcccctata gtgagtcgta ttaatttcga ttatgcggcc gtgtacaata cgattacttt
      241 ctgttcgact taagcattat gcggccgcaa gcttggatcc tcattcattc agtgatgatg
      301 atggtgatga gaagaacctc aggctttatt gtccactttg ccgcccgctt cgtcacgtaa
      361 ttctcgtcgc aaaatttttc cgacgttaga tttcggtaac tcatcacgaa actccaccag
      421 cttcggtact ttgtagcccg tgagctgacg gcggcaaaag gtcaccagtg actcttcggt
      481 aagcgatgga tcttttttca ctacgaagat tttcaccgct tcaccactgg agccggaagg
      541 tacgccaaca gccgcgactt cctgtacgcc aggatgctgc atgacgacat cttcaatctc
      601 gttgggatag acgttaaaac cggaaaccag aatcatgtct tttttacgat cgacaatgcg
      661 caggaaccct tcttcatcca tcaccgcgat gtcgccggtg tgtaaccagc catttttgat
      721 gatctcatct gtagcatccg gacgctgcca gtaacccagc atcacctgcg gtcctttgac
      781 acaaagctca cccggttgac ccggtggtac ttcattatca tcatcatcca ccagtttggc
      841 ttccgtcgac ggcaccggca aaccgatgct accactatga taatcaatat catatgggtt
      901 aacgctgacc agcggcgcac actcggtaag gccatagcct tccagcagat actgtcctgt
      961 cagtttcacc caacgctctg ccaccacttg ctgcactggc atccctccgc ctgcggaaag
     1021 atgcaaactg gagaaatcca gctgctggaa ctccttattg ttcagca
//
