LOCUS       sequence-114871-        1107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD V451G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS522 (TJF089)
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..47)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    156..180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(180..199)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(181..199)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(288..305)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gacgagtcca tgtgctggcg ttcaaatttc gcagcagcgg tttctttacc agactcgagg
       61 gtaccgacgt cagcgatcgc gtggccggcc gatatccaat tgagatctgc catatgtata
      121 tctccttctt atacttaact aatatactaa gatggggaat tgttatccgc tcacaattcc
      181 cctatagtga gtcgtattaa tttcgattat gcggccgtgt acaatacgat tactttctgt
      241 tcgacttaag cattatgcgg ccgcaagctt ggatcctcat tcattcagtg atgatgatgg
      301 tgatgagaag aacctcaggc tttattgtcc actttgccgc gcgcttcgtc acgtaattct
      361 cgtcgcaaaa tttttccgac gttagatttc ggtaactcat cacgaaactc caccagcttc
      421 ggtactttgt agcccgtgag ctgacggcgg caaaaggtca ccagtgactc ttcggtaagc
      481 gatggatctt ttttcactac gaagattttc accgcttcac cactggagcc ggaaggtacg
      541 ccaacagccg cgacttcctg tacgccagga tgctgcatga cgacatcttc aatctcgttg
      601 ggatagacgt taaaaccgga aaccagaatc atgtcttttt tacgatcgcc aatgcgcagg
      661 aatccttctt catccatcac cgcgatgtcg ccggtgtgta accagccatt tttgatgatc
      721 tcatctgtag catccggacg ctgccagtaa cccagcatca cctgcggtcc tttgacacaa
      781 agctcacccg gttgacccgg tggtacttca ttatcatcat catccaccag tttggcttcc
      841 gtcgacggca ccggcaaacc gatgctacca ctatgataat caatatcata tgggttaacg
      901 ctgaccagcg gcgcacactc ggtaaggcca tagccttcca gcagatactg tcctgtcagt
      961 ttcacccaac gctctgccac cacttgctgc actggcatcc ctccgcctgc ggaaagatgc
     1021 agactggaga aatccagctg ctggaactct ttattgttca gcaacgcatt gaaaagggtg
     1081 ttaacgccgg tgatagcggt aaacgga
//
