LOCUS       sequence-114882-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intron targeting-mediated EGFP knockin at the zebrafish th locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Li J, Zhang BB, Ren YG, Gu SY, Xiang YH, Huang C, Du JL
  TITLE     Intron targeting-mediated and endogenous gene integrity-maintaining 
            knockin in zebrafish using the CRISPR/Cas9 system.
  JOURNAL   Cell Res. 2015 Apr 7. doi: 10.1038/cr.2015.43.
  PUBMED    25849248
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2015 Apr 7. doi: 10.1038/cr.2015.43."
COMMENT     Sequence Label: th-P2A-EGFP donor
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     126..145
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     385..406
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(452..508)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
       61 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
      121 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
      181 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
      241 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
      301 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
      361 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
      421 cggtgaacag ctcctcgccc ttgctcacca taggaccagg attttcttca acatcaccag
      481 cttgcttaag caaggagaaa ttagtagctc cggatccagc caacacattc agggcatctg
      541 tgagaatctt cagttcatct tttatggtct ccaggccttt ctggatcttc agtgggttgt
      601 ccagcacttc tatgctgtcc gtgtacggat cgaaccgcac agaaaacggt cgcttgatgc
      661 gggtcacata tgttctgtaa ataataatta acaaataaaa aacatttaag aaataaaaat
      721 gtgattagtc aattcatgct gttttaaggg atatgtcttt aaaaaaagtt aattgcattt
      781 ttttaaaagc tagttcaaaa aaatgatagt tgacatactg tgaaaacttc agaaagtttt
      841 atctcattat cgcatctttt tgctttgtga ttttaaaact atttttaata agaataaaat
      901 ctatattttt tattattgtt attatcgctg atattattat tataattgtg gttgttgttg
      961 tgt
//
