LOCUS       sequence-114886-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intron targeting-mediated EGFP knockin at the zebrafish th locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Li J, Zhang BB, Ren YG, Gu SY, Xiang YH, Huang C, Du JL
  TITLE     Intron targeting-mediated and endogenous gene integrity-maintaining 
            knockin in zebrafish using the CRISPR/Cas9 system.
  JOURNAL   Cell Res. 2015 Apr 7. doi: 10.1038/cr.2015.43.
  PUBMED    25849248
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2015 Apr 7. doi: 10.1038/cr.2015.43."
COMMENT     Sequence Label: th-P2A-EGFP donor
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctcggtaccg cgattacatg gcgtagtacg ctatttatat tctcctggat attgtataag
       61 tgtggtggtg cttgcttctg atttttatat aatccattaa catatttata cacatagcta
      121 ggcctgttta taaactttta tgttcaaaaa agcttgtaca catggatgta ttaaaagaac
      181 ttttattacc tgctgccttt tcttccgttt acacagtttt ttttttactt tctccccttt
      241 atgagtgtaa caacccctgg gtagtctagg ggagtggggt tgcacacttg accgaagatt
      301 ttgttcaaat aaacgctcaa agaaaattgt actcaaagta atgaccacat acaagaattg
      361 ctgtgcaaat aaagtgattt attacaggtg gtgataagtg aagcagtgag tgagctattt
      421 acaatattta caattcacaa ccaaaacaaa acataaatta gaaagactgg ctatgggtgg
      481 cgctaaagca aagaaacaat caaaaccaaa cagtcttact aatatcaaat ccctgctaat
      541 ctagtctaat aaacaataat gtgaaaaata aatcttgatt gtccaagatg tcttacctaa
      601 actacctaaa aacgtttgga agtctataaa agttgttcag catttcttat tttgatcaat
      661 ttaaacaatt ataaagaaca caaaacataa acattttgat gttatgatag caattccaat
      721 gtagaagaat cactttctgc cctccataca caaataaatt cagatggagg ctccatctga
      781 attttgtgcg tcaacaatga cgtcatgtga aaacaaccta ttgcatgcat ttggaaatgt
      841 acaataacat tgcaacaacc aacacaacaa caaccacaat tataataata atatcagcga
      901 taataacaat aataaaaaat atagatttta ttcttattaa aaatagtttt aaaatcacaa
      961 agcaaaaaga tgcgataatg agataaaact ttctgaagtt ttc
//
