LOCUS       sequence-114887-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression vector of Rif1 siRNA resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Escribano-Diaz C, Orthwein A, Fradet-Turcotte A, Xing M, Young JT, 
            Tkac J, Cook MA, Rosebrock AP, Munro M, Canny MD, Xu D, Durocher D
  TITLE     A cell cycle-dependent regulatory circuit composed of 53BP1-RIF1 and
            BRCA1-CtIP controls DNA repair pathway choice.
  JOURNAL   Mol Cell. 2013 Mar 7;49(5):872-83. doi: 
            10.1016/j.molcel.2013.01.001. Epub 2013 Jan 17.
  PUBMED    23333306
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.01.001"; journalName: "Mol Cell"; date: 
            "2013-03-7- 7"; volume: "49"; issue: "5"; pages: "872-83"
COMMENT     Sequence Label: pDEST pcDNA5-FRT/TO-eGFP-Rif1
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(57..59,60..62,63..773)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(57..59,60..62,63..773)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(102..123)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(363..382)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     710..731
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccatgg
       61 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      121 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      181 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      241 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
      301 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      361 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      421 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      481 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
      541 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      601 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      661 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      721 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagg
//
