LOCUS       sequence-114895-        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intron targeting-mediated EGFP knockin at the zebrafish th locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Li J, Zhang BB, Ren YG, Gu SY, Xiang YH, Huang C, Du JL
  TITLE     Intron targeting-mediated and endogenous gene integrity-maintaining 
            knockin in zebrafish using the CRISPR/Cas9 system.
  JOURNAL   Cell Res. 2015 Apr 7. doi: 10.1038/cr.2015.43.
  PUBMED    25849248
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2015 Apr 7. doi: 10.1038/cr.2015.43."
COMMENT     Sequence Label: th-P2A-EGFP donor
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(760..781)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac atgggaacct cctagtacct
       61 ttaaggtaca aaagtgtacc ctaaagattc taatgtccat ctttatttta caaaaatgta
      121 ttttttgaaa aagtaccacc tcagtgacag attttgtatg tttatttgta tgttggagta
      181 tatatattgt aaacttagga tctgctttta ggcttaatat gtattgtgaa tatgacctct
      241 ggtaatattt catatatacc ttttgactta aaaagcttta ataatgaggg tacgtctatc
      301 tgaaatataa ataaatcctg tacataaatt aattaaatgt catatcagaa atttctgttt
      361 agacgagtta taatttaccc caaaatgaaa attatagcgg aaaatgttat cattttaaat
      421 tttgatcata tatgacttaa aaaaaaaaat aaaaaaagtt cattcagaaa taaaagtctc
      481 tctctgtctc tctctttctc tatctctatt atcatattct actgtaatgt gagtttgagt
      541 ggtgaacttt catttttgga tacattattt aacgctgaag gtttaaagtt tttcattcag
      601 tattttagaa gcaacaatgt ctatagcagc agatgtacag tagacataga agcacagcat
      661 aacaatgaga ccgtcacaca tgaagcatag aggaatgatt aagcagaatt aaccggtctt
      721 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
      781 gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt
      841 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
      901 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt
      961 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
     1021 caggatgttg ccgtcctcct tgaag
//
