LOCUS       sequence-11490-e        7065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Schowalter RM, Pastrana DV, Pumphrey KA, Moyer AL, Buck CB.
  TITLE     Merkel cell polyomavirus and two previously unknown polyomaviruses 
            are chronically shed from human skin.
  JOURNAL   Cell Host Microbe. 2010 Jun 25;7(6):509-15.
  PUBMED    20542254
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2010-06-25"; volume: "7(6)"; pages: "509-15"
COMMENT     Sequence Label: pHPyV6-607a
FEATURES             Location/Qualifiers
     source          1..7065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     176..198
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     190..207
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     191..207
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    223..322
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(3377..3388)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5258..5357)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(5954..5972)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5964..6011)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      complement(6097..6685)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6177..6196)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6893..6910)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      join(7063..7065,1..84)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
       61 aacgctctcc tgagtaggac aaatccgccg ggagaacaac aacaattgca ttcattttat
      121 gtttcaggtt cagggggagg tgtgggaggt tttttaacgc tagcatggat gttttcccag
      181 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt
      241 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
      301 actttgtaca aaaaagcagg ctcaaaagct tcccagagta ataaaaaaag gaggagtgga
      361 ggttatggaa acagtgccac tttctgaaga cacaatttat aaagtggagg caattctgct
      421 gcctaatttt gcttctggat ccaatactgc agtataccag tctcggggag ccccctacac
      481 ctttactgac actttggatg ctggttcatc tctgtgttat accctggctg ttgttaattt
      541 gcctgaaatt cctgaggccc tgtgtgatga tactttgttg gtttgggaag ccttcagagt
      601 tgaaacagaa ctaatattta ccccacaagt gggaagtgct ggatatataa gagcacaagg
      661 aacccctgct ggtgtagaag gttcccaaat gtacttttgg gcctgtggag gcagcccctt
      721 ggatgtcatt ggtattaacc cagatccaga aagaatgaat gtagcagcag gcctggaagg
      781 gcctagtaaa gagaaccaac catctgttgc aggcattaaa gctaccagaa aacaagttac
      841 agctgccaat tttcccattg aaatttggag tgctgatcct accaggaatg agaactgtag
      901 atattttggc agaattgttg gtggtagtgt tacccctcca gtggtttcct ttggaaatca
      961 gagcaccact ccattggttg atgaaaatgg agtaggtata ctctgtttgt ttggagctat
     1021 atatctaaca tcagcagata tgttggggat ggtagggtat gctggtaacc ccactctcag
     1081 tgatgcctat agccagcaaa gaagtgtgca ggctgccttt ggaagatttt ttagagttca
     1141 cttcagacag aggagagtta aacatccata tactgtggat atgatgttta gacaattcct
     1201 gcagcctcag aagccacagg tgcaagggac acaacctaat gctgtccaag aagtagtaat
     1261 ggaacaaatg cagccaagca tacttccaac tactctggag ggtgctattg ggtactcacc
     1321 cagtacaaag tttattctcc aaaatgggga acttatatac ccttctagta cagtagcagc
     1381 aggtgctgca aatttgtttg ggcctccagt agagaaacag acaagcaagg aacctagtaa
     1441 gggtgagctg taaacccctc tttctgtgaa agacaaagca tatgctgggt aattgttaaa
     1501 caatgaattc tttattgtat caataaatct tacatggaat gcatggagcc aatgccagat
     1561 tcttgagtac tgtcatttgt ttcatcattt tcctgtggct gtacaaaaga ttcatctacc
     1621 agtacatttg taaaagggtc aatgccagct aaaatatttt ctttcatagt ttggtacatt
     1681 ccaaagctaa cccatctttc aaaagtttgc ttccagttaa caacatcttc ctgcagttct
     1741 tccataaagt cctccactgg ctcccaccaa attagagctg ccaacaaggt ggtccccttt
     1801 tgtaacagcc ttttggacat taatacacaa tctttcaaac atttttgcaa acatggctta
     1861 tgcagaaaat aatatcctct ggcaattctg gcctttacag tacatggaat tatatagtca
     1921 ttacaggtga tcacagatgg aggaaacagc tgggacacct tgtttagatg cttcctctcc
     1981 atatttactg gcacacatcc atctaaataa tccctaaggt tatctaggtt tgtcatccca
     2041 cacccttgtg taagattggt attagccata ggtgttccct ttacatcctc aatgacacac
     2101 atgaatttgt caatggcaca tcccagttca aagtttatct tatctggagt acaatttaca
     2161 ttcaaagaca caccccctac aagatccaat attgcagcag ccaatgttgt tttgccacta
     2221 tttatggggc ctttgaacaa cacatttctt tttttaggta tgttttcagt taaaatgctt
     2281 agtatttcct ttagtttagt agtaaagcta ggctgcaaac aatctaacca ggccacagcc
     2341 cccagtaatt gagtaatttc cacacctcct gcatattgct cagctaggat tgtaaattgc
     2401 ttcttaaagg cctgtaatac cagatcctgt ctagttgatt ccaacattaa cacccttctt
     2461 tgtgctgcta ctctgtcaca ggcctgctga gcaatagatt tctggttttt agctgctttg
     2521 aataattggg cattgtgaaa gtgtacttca tggtgtttat gcactttcac tcccttttta
     2581 catttcatac agcttgctgg atcattggca aattctatat aatatcccaa taatgccaga
     2641 caatcagtta ggtctgtgtc actagcaaaa gaagctactt gattccaatc acattcctct
     2701 tttttggagc cttcctgaaa gtcataactg tgtaaaccct cagccctaga ttcttgtata
     2761 acagtaaatt catccccctg caaggccata taacattcag cagattttaa aacagctttg
     2821 attaacagaa atgaaatagt acagcatttt gacaaagcat ttagcatagc agacactcta
     2881 tgtcttctag gagtaataca gtaaagtata gcccctgtat tatcattgca tttataggcc
     2941 ccagtaaaaa ctgcattaaa tgctgactgt agcttatcat acaaggtttc ccatttttca
     3001 agggttgtat agctaacaaa gcaggtatag catttattac caagagttgc atggcttaga
     3061 taatcatgca ggcaagatgg aaagtcattt ggagcaggat tgggttttct tttcttagga
     3121 ggagtgcaag aatacttgct gtcttgagag ttgccttcac cagggtccac attgtcttct
     3181 tcttctgagg ctgacaggtg ttcatcacaa tataaatctt catcgtccca gcctctattg
     3241 aaatcagccc accattgttc ccagccggga ctgccatatt gtggaggtgg ccttacctgt
     3301 aaaataaatt ataagtttca atgcatcact acctggacta tctataaaaa ttgcacttac
     3361 caccagatga gcttctctgt aattcccaac caatccaggg ggctttggta gataatgtgg
     3421 gtccaccaca taaaagaatc agcttccaca ggtaggccaa accagagaca gtagcacttg
     3481 tagcaccagc actttcccca aatagttggc tttcctaaat taatctccac ttgcttttta
     3541 tgctcctttt tgagaaggca atgaaggcat ttgcaggaac tgattccttg ttgtacacac
     3601 aaacggaaat cccaaacttt ctttttatga aattcagggc ctaagaactc cccaacagtt
     3661 aagggaatac cccaatcatc actgacctca gaggaaaagc accaggtggg tgatgaagac
     3721 atttggtccc tcagggtggc attcaacttc tcctttaaaa cattcaggcg ttgcattttt
     3781 tcaggatccc ctcctttatc aggatgatat tttttacaag ctagtcttat cttctgttgc
     3841 ataaggggta aattacccca acaggccatg ctgaggccta taagatccat tagctcccta
     3901 acttcttctc tggctaaaag gcgatccatt ttgctgcttg agtagtctct gaatgaaatg
     3961 ttaaaatccc agacagcagg tgaaatccct gtgcaggctt acgcaactgg gcagggccat
     4021 ttaagctccc ttatctctct aattatagga ggcaataaag gccaccaggc cacctcaatg
     4081 tatgagaaaa aaggagagga aatagggcaa ccaaaggtca aaaggaagtt attaggcgag
     4141 aaaagcccgc caaatttttt cccagtcata actgaggttg accaccgttg acacaacacc
     4201 ctaattagta agttcttcaa tttctgtttt attttgtttt aactcttcag tgacctcacc
     4261 gcctgttgac cctggtctta atttctactt aacaggtaag ccatgggaat tctatttagt
     4321 cttcctgaaa ttatagcagc tgctgctgtt ggagggggag aggccctgga aattgctggt
     4381 ggtctaggag cccttgtatc aggggaagga ctggctacat tagaggctct acagtctgct
     4441 gctgctttat ccagtgaagc aactgcagct ctagctgtat ctaatgaggc tgccattgta
     4501 ttgtcaacag ttcctgagtt gtcccagacc ctctttggag cacaattact actgtcttct
     4561 gtagcaggcg ttggaggtgt tatatacagc aactataacc ctggggaact gtacaaggcg
     4621 ccagaaggac caggtggact cggcccaaga gttggaaaca ccaccatggc acttcaactg
     4681 tggttgccac aagtttggtc ttggggaggc gcgggtagag gtctccccga ctggcttatc
     4741 aacatgcttc gtgaagtgcc gtctcccaca gagatcctca gtgacattgt cagaggaata
     4801 tggacttcct actatagagc aggcagggag ataatccaaa gaacagcttc tagggaattg
     4861 ggggccttgc tgagtagggt aagagaaact gttatacatg gggctgaaag agctttggaa
     4921 gcagcccctg acccagtgca aggcctagta aatctagtaa actatgctgt aaattataat
     4981 agacagtggg aaacaagggc cctgttagaa ggaaggcctc tgtttgaggg caatggagta
     5041 gtaaactatg atatgcaaaa tcttcctgta aatggaaata atgatcaaag gggggggttt
     5101 catgatgaag gtctttgggt aagtttctca gcagagcagg gaaatactgg ccaatactgt
     5161 atacctcaat ggctgctttt tgttcttgag gagctggaca aagagattaa ggaagatgcc
     5221 ttgtcacaga aaaggaaatg gaccaattca aaagcttacc cagctttctt gtacaaagtt
     5281 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     5341 aataaaatca ttatttgcca tccagctgat aagcaagtaa aacctctaca aatgtggtaa
     5401 aatcgataag gatccgggct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     5461 acagttgcgt acctaaccaa gttcctcttt cagaggttat ttcaggccct gcagggttaa
     5521 ttaacaaaaa accccgccgg ggcggggttt tttgttacaa ttcttatcct cagtcctgct
     5581 cctctgccac aaagtgcaca cagttgcctg ctgggtctct cagggcaaac tctctccccc
     5641 agggctgctc tccaatctct gtcatggcag ggccactggc atccctgaag ttggtggaga
     5701 ccacctcact ccactcagca tacagctcat ccagtcctct cacccacacc caagccaggg
     5761 tgttgtcagg caccacctgg tcctggactg ctgagatgaa cagggtgaca tcatctctga
     5821 ccacacctgc aaagtcatcc tccacaaaat ctctggagaa ccccaacctg tcagtccaga
     5881 actcaacagc tccagccaca tccctggctg tgagcactgg gacagcactg gtcaacttgg
     5941 ccatgatggc cctcctatag tgagtcgtat tatactatgc cgatatacta tgccgatgat
     6001 taattgtcaa ctacttttga aaaatttaat taagaacatg tgagcaaaag gccagcaaaa
     6061 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6121 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6181 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6241 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6301 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6361 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6421 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6481 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     6541 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6601 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6661 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6721 tcagtggaac gaaaactcac gttaagggat tttggtcatg gctagttaat taacccacgt
     6781 ttcaacatgc acatcccagt aatttggaaa cattttgttt ccaaagattc acttaacatt
     6841 ggtttagcaa catgaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac
     6901 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
     6961 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
     7021 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaa
//
