LOCUS       sequence-114915-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD Y9H-His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS535 (TJF078)
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    159..183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(183..202)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(184..202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(291..308)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagacgagtc catgtggctg gcgttcaaat ttcgcagcag cggtttcttt accagactcg
       61 agggtaccga cgtcagcgat cgcgtggccg gccgatatcc aattgagatc tgccatatgt
      121 atatctcctt cttatactta actaatatac taagatgggg aattgttatc cgctcacaat
      181 tcccctatag tgagtcgtat taatttcgat tatgcggccg tgtacaatac gattactttc
      241 tgttcgactt aagcattatg cggccgcaag cttggatcct cattcattca gtgatgatga
      301 tggtgatgag aagaaccggc tttattgtcc actttgccgc gcgcttcgtc acgtaattct
      361 cgtcgcaaaa tttttccgac gttagatttc ggtaactcat cacgaaactc caccagcttc
      421 ggtactttgt agcccgtgag ctgacggcgg caaaaggtca ccagtgactc ttcggtaagc
      481 gatggatctt ttttcactac gaagattttc accgcttcac cactggagcc ggaaggtacg
      541 ccaacagccg cgacttcctg tacgccagga tgctgcatga cgacatcttc aatctcgttg
      601 ggatagacgt taaaaccgga aaccagaatc atgtcttttt tacgatcgac aatgcgcagg
      661 aacccttctt catccatcac cgcgatgtcg ccggtgtgta accagccatt tttgatgatc
      721 tcatctgtag catccggacg ctgccagtaa cccagcatca cctgcggtcc tttgacacaa
      781 agctcacccg gttgacccgg tggtacttca ttatcatcat catccaccag tttggcttcc
      841 gtcgacggca ccggcaaacc gatgctacca ctatgataat caatatcata tgggttaacg
      901 ctgaccagcg gcgcacactc ggtaaggcca tagccttcca gcagatactg tcctgtcagt
      961 ttcacccaac gctctgccac cacttgctgc actggcatcc ctccgcctgc ggaaagatgc
     1021 aaactggaga aatccagctg
//
