LOCUS       sequence-114916-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intron targeting-mediated Gal4 knockin at the zebrafish th locus. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Li J, Zhang BB, Ren YG, Gu SY, Xiang YH, Huang C, Du JL
  TITLE     Intron targeting-mediated and endogenous gene integrity-maintaining 
            knockin in zebrafish using the CRISPR/Cas9 system.
  JOURNAL   Cell Res. 2015 Apr 7. doi: 10.1038/cr.2015.43.
  PUBMED    25849248
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2015 Apr 7. doi: 10.1038/cr.2015.43."
COMMENT     Sequence Label: th-P2A-Gal4 donor
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(127..183)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gggagagtag cgacactccc agttgttctt cagacacttg gcgcacttcg gtttttcttt
       61 ggagcacttg agctttttaa gtcggcaaat atcgcatgct tgttcgatag aagacagtag
      121 cttcatagga ccaggatttt cttcaacatc accagcttgc ttaagcaagg agaaattagt
      181 agctccggat ccagccaaca cattcagggc atctgtgaga atcttcagtt catcttttat
      241 ggtctccagg cctttctgga tcttcagtgg gttgtccagc acttctatgc tgtccgtgta
      301 cggatcgaac cgcacagaaa acggtcgctt gatgcgggtc acatatgttc tgtaaataat
      361 aattaacaaa taaaaaacat ttaagaaata aaaatgtgat tagtcaattc atgctgtttt
      421 aagggatatg tctttaaaaa aagttaattg cattttttta aaagctagtt caaaaaaatg
      481 atagttgaca tactgtgaaa acttcagaaa gttttatctc attatcgcat ctttttgctt
      541 tgtgatttta aaactatttt taataagaat aaaatctata ttttttatta ttgttattat
      601 cgctgatatt attattataa ttgtggttgt tgttgtgttg gttgttgcaa tgttattgta
      661 catttccaaa tgcatgcaat aggttgtttt cacatgacgt cattgttgac gcacaaaatt
      721 cagatggagc ctccatctga atttatttgt gtatggaggg cagaaagtga ttcttctaca
      781 ttggaattgc tatcataaca tcaaaatgtt tatgttttgt gttctttata attgtttaaa
      841 ttgatcaaaa taagaaatgc tgaacaactt ttatagactt ccaaacgttt ttaggtagtt
      901 taggtaagac atcttggaca atcaagattt atttttcaca ttattgttta ttagactaga
      961 ttagcaggga tttgatatta gtaagactg
//
