LOCUS       sequence-114918-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD D372G-His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS534 (TJF077)
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..52)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    161..185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(185..204)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(186..204)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(293..310)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagtagacga gtccatgtgc tggcgttcaa atttcgcagc agcggtttct ttaccagact
       61 cgagggtacc gacgtcagcg atcgcgtggc cggccgatat ccaattgaga tctgccatat
      121 gtatatctcc ttcttatact taactaatat actaagatgg ggaattgtta tccgctcaca
      181 attcccctat agtgagtcgt attaatttcg attatgcggc cgtgtacaat acgattactt
      241 tctgttcgac ttaagcatta tgcggccgca agcttggatc ctcattcatt cagtgatgat
      301 gatggtgatg agaagaaccg gctttattgt ccactttgcc gcgcgcttcg tcacgtaatt
      361 ctcgtcgcaa aatttttccg acgttagatt tcggtaactc atcacgaaac tccaccagct
      421 tcggtacttt gtagcccgtg agctgacggc ggcaaaaggt caccagtgac tcttcggtaa
      481 gcgatggatc ttttttcact acgaagattt tcaccgcttc accactggag ccggaaggta
      541 cgccaacagc cgcgacttcc tgtacgccag gatgctgcat gacgacatct tcaatctcgt
      601 tgggatagac gttaaaaccg gaaaccagaa tcatgtcttt tttacgatcg acaatgcgca
      661 ggaacccttc ttcatccatc accgcgatgt cgccggtgtg taaccagcca tttttgatga
      721 tctcatctgt agcatccgga cgctgccagt aacccagcat cacctgcggt cctttgacac
      781 aaagctcacc cggttgaccc ggtggtactt cattatcatc atcatccacc agtttggctt
      841 ccgtcgacgg caccggcaaa ccgatgctac cactatgata atcaatacca tatgggttaa
      901 cgctgaccag cggcgcacac tcggtaaggc catagccttc cagcagatac tgtcctgtca
      961 gtttcaccca acgctctgcc accacttgct gcactggcat ccc
//
