LOCUS       sequence-114919-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intron targeting-mediated Gal4 knockin at the zebrafish th locus. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Li J, Zhang BB, Ren YG, Gu SY, Xiang YH, Huang C, Du JL
  TITLE     Intron targeting-mediated and endogenous gene integrity-maintaining 
            knockin in zebrafish using the CRISPR/Cas9 system.
  JOURNAL   Cell Res. 2015 Apr 7. doi: 10.1038/cr.2015.43.
  PUBMED    25849248
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2015 Apr 7. doi: 10.1038/cr.2015.43."
COMMENT     Sequence Label: th-P2A-Gal4 donor
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(927..943)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(927..943)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(940..962)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(975..981,982..999,1000..1005))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1020..1041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcgtcgagat cgaccccggg aattcagatc tctcgagccg cccccccgac cgatgtcagc
       61 ctgggggacg agctccactt agacggcgag gacgtggcga tggcgcatgc cgacgcgcta
      121 gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg atttaccccc
      181 cacgactccg ccccctacgg cgctctggat atgaccggtt aattctgctt aatcattcct
      241 ctatgcttca tgtgtgacgg tctcattgtt atgctgtgct tctatgtcta ctgtacatct
      301 gctgctatag acattgttgc ttctaaaata ctgaatgaaa aactttaaac cttcagcgtt
      361 aaataatgta tccaaaaatg aaagttcacc actcaaactc acattacagt agaatatgat
      421 aatagagata gagaaagaga gagacagaga gagactttta tttctgaatg aacttttttt
      481 attttttttt ttaagtcata tatgatcaaa atttaaaatg ataacatttt ccgctataat
      541 tttcattttg gggtaaatta taactcgtct aaacagaaat ttctgatatg acatttaatt
      601 aatttatgta caggatttat ttatatttca gatagacgta ccctcattat taaagctttt
      661 taagtcaaaa ggtatatatg aaatattacc agaggtcata ttcacaatac atattaagcc
      721 taaaagcaga tcctaagttt acaatatata tactccaaca tacaaataaa catacaaaat
      781 ctgtcactga ggtggtactt tttcaaaaaa tacatttttg taaaataaag atggacatta
      841 gaatctttag ggtacacttt tgtaccttaa aggtactagg aggttcccat gtcgacctgc
      901 aggcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      961 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     1021 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttc
//
