LOCUS       sequence-114921-        1118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD F447D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS514 (TJF081)
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..52)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    161..185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(185..204)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(186..204)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(293..310)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagtagacga gtccatgtgc tggcgttcaa atttcgcagc agcggtttct ttaccagact
       61 cgagggtacc gacgtcagcg atcgcgtggc cggccgatat ccaattgaga tctgccatat
      121 gtatatctcc ttcttatact taactaatat actaagatgg ggaattgtta tccgctcaca
      181 attcccctat agtgagtcgt attaatttcg attatgcggc cgtgtacaat acgattactt
      241 tctgttcgac ttaagcatta tgcggccgca agcttggatc ctcattcatt cagtgatgat
      301 gatggtgatg agaagaacct caggctttat tgtccacttt gccgcgcgct tcgtcacgta
      361 attctcgtcg caaaattttt ccgacgttag atttcggtaa ctcatcacga aactccacca
      421 gcttcggtac tttgtagccc gtgagctgac ggcggcaaaa ggtcaccagt gactcttcgg
      481 taagcgatgg atcttttttc actacgaaga ttttcaccgc ttcaccactg gagccggaag
      541 gtacgccaac agccgcgact tcctgtacgc caggatgctg catgacgaca tcttcaatct
      601 cgttgggata gacgttaaaa ccggaaacca gaatcatgtc ttttttacga tcgacaatgc
      661 gcagatctcc ttcttcatcc atcaccgcga tgtcgccggt gtgtaaccag ccatttttga
      721 tgatctcatc tgtagcatcc ggacgctgcc agtaacccag catcacctgc ggtcctttga
      781 cacaaagctc acccggttga cccggtggta cttcattatc atcatcatcc accagtttgg
      841 cttccgtcga cggcaccggc aaaccgatgc taccactatg ataatcaata tcatatgggt
      901 taacgctgac cagcggcgca cactcggtaa ggccatagcc ttccagcaga tactgtcctg
      961 tcagtttcac ccaacgctct gccaccactt gctgcactgg catccctccg cctgcggaaa
     1021 gatgcagact ggagaaatcc agctgctgga actctttatt gttcagcaac gcattgaaca
     1081 aggtgtttac gccggtgata gcggtaaacg gatatttc
//
