LOCUS       sequence-114928-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for bacterial overexpression of HuD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  TITLE     Identification of the transcriptome-wide mRNA targets of the 
            neuronal ELAVL proteins (unpublished)
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET23a_mod_HuD_FL
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..26)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg cggacttgtg ggctttgttg
       61 gttttaaagg aaacttgcaa cactctgtct cccaggcggt acccgttgag gctggcgatg
      121 gccatggccg cctcatcata gttggtcatg gtgacaaagc cgaatccctt gcacttgttg
      181 gtgttgaagt cacgaatcac ctttacgttg ttcactgctc caaaggggcc aaagagctgc
      241 cagaggacac tctcatcgga atcgggggac aggttgtaga caaagatgca ccacccagtt
      301 cctgtgtgac cagggatgtt cattcccaca aggcttgtca ttccatcaat ggtaattggg
      361 gagaacctct ttacgccata ggccatatta agcaaattgt ccagcctgaa cctctgagcc
      421 tggtggtgaa gtggacctgg gtagcgccgg ttaggggact ggtagagctg ggagagcagg
      481 gcctggctgg acttctggct ggggttgttg gcaaacttca cagtaatcgg ttccgtagca
      541 ccgctgggct tctggccatt cagccctttg atggcttctt ctgcctcaat cctcttatca
      601 aagcggatga atcccacccc tctggacact cctgtgactt gatcaaccag gattcgtgag
      661 gtgatgatac ggccgtattg cgagaaaagt tgctccagtt ccttctgggt catggttttg
      721 ggaag
//
