LOCUS       sequence-114930-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutively expresses FLAG-HA-tagged RBPMS2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pFRT-DestFLAGHA_RBPMS2
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             173..196
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             197..223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    239..263
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgttttagt
       61 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
      121 ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtacca tggactacaa
      181 ggacgacgat gacaagtacc cttatgacgt gcccgattac gctttgaacc agatatcaac
      241 aagtttgtac aaaaaagcag gcttcatgag caacctgaag ccggacggcg agcacggcgg
      301 cagcaccggc accggctccg gcgcgggctc cggcggcgcc ctggaggagg aggtccggac
      361 actgtttgtc agcggcctcc ctgtggacat taaacccaga gaactctact tgctcttccg
      421 gccgttcaag gggtatgaag ggtccctgat caagctcact gcaagacagc ctgttggttt
      481 tgtgatcttt gacagccgtg caggagcaga agcggccaag aatgcgctga acggtattcg
      541 ctttgatccc gaaaatccac agactctgag gctagagttt gccaaagcca acaccaagat
      601 ggccaagagc aagctaatgg caactccaaa tcccagcaac gtgcaccccg ccctaggagc
      661 acacttcatc gcacgggacc cctatgacct gatgggggct gctctgatcc ctgcatcccc
      721 agaggcctgg gccccctacc ctttgtacac cacagagctg accccagcca tctcccatgc
      781 tgcgttcacc tacccaactg ccactgccgc tgccgccgcc ctccacgctc
//
