LOCUS       sequence-114934-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses RFP-tagged RBPMS in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pFRT-TODestRFP_RBPMS
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(399..417)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     667..686
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgttttagt gaaccgtcag
       61 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      121 agcctccgga ctctagcgtt taaacttaag cttggtaccg agctcggatc catggtgagc
      181 aagggcgagg ggtaccatgg tgagcaaggg ggtaccatgg tgagcaaggg cgggggtacc
      241 aggtgagcaa gggcgagggg gtaccatggt gagcaagggc gaggaggtca tcaaagagtt
      301 catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      361 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      421 cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc
      481 gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt
      541 caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc
      601 ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc
      661 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
      721 cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga
//
