LOCUS       sequence-114939-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP 
            expression cassette - Ampicillin plasmid 
            [E:J23102:B:BCD2:C:E0040m:D:B0015:F].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: pJ02B2Gm_EF
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    4..24
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(42..69)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(85..156)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(219..239)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 agcctgcagc ggccgctact agtaggtctc tagcgtataa acgcagaaag gcccacccga
       61 aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga taaaacgaaa
      121 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggacctac ctttattatt
      181 tgtatagttc atccatgcca tgtgtaatcc cagcagctgt tacaaactca agaaggacca
      241 tgtgatctct cttttcgttg ggatctttcg aaagggcaga ttgtgtggac aggtaatggt
      301 tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt ttgttgataa tggtctgcta
      361 gttgaacgct tccatcttca atgttgtgtc taattttgaa gttaactttg attccattct
      421 tttgtttgtc tgccatgatg tatacattgt gtgagttata gttgtattcc aatttgtgtc
      481 caagaatgtt tccatcttct ttaaaatcaa taccttttaa ctcgattcta ttaacaaggg
      541 tatcaccttc aaacttgact tcagcacgtg tcttgtagtt cccgtcatct ttgaaaaata
      601 tagttctttc ctgtacataa ccttcgggca tggcactctt gaaaaagtca tgctgtttca
      661 tatgatctgg gtatctcgca aagcattgaa caccataacc gaaagtagtg acaagtgttg
      721 gccatggaac aggtagtttt ccagtagtgc aaataaattt aagggtaagt tttccgtatg
      781 ttgcatcacc ttcaccctct ccactgacag aaaatttgtg cccattaaca tcaccatcta
      841 attcaacaag aattgg
//
