LOCUS       sequence-114942-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP 
            expression cassette - Ampicillin plasmid 
            [E:J23102:B:BCD2:C:E1010m:D:B0015:F].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: pJ02B2Rm_EF
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    4..24
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(42..69)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(85..156)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(172..849)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 agcctgcagc ggccgctact agtaggtctc tagcgtataa acgcagaaag gcccacccga
       61 aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga taaaacgaaa
      121 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggaccttt attaagcacc
      181 ggtggagtga cgaccttcag cacgttcgta ctgttcaacg atggtgtagt cctcgttgtg
      241 ggaggtgatg tccagtttga tgtcggtttt gtaagcaccc ggcagctgaa ccggtttttt
      301 agccatgtag gtggttttaa cttcagcgtc gtagtgacca ccgtctttca gtttcagacg
      361 cattttgatt tcacctttca gagcaccatc ctccgggtac atacgttcgg tggaagcttc
      421 ccaacccatg gtttttttct gcataaccgg accgtcggac gggaagttgg taccacgcag
      481 tttaactttg tagatgaact caccgtcttg cagggaggag tcctgggtaa cggtaacaac
      541 accaccatct tcgaagttca taacacgttc ccatttgaaa ccttccggga aggacagttt
      601 caggtagtcc gggatgtcag ccgggtgttt aacgtaagct ttggaaccgt actggaactg
      661 cggggacagg atgtcccaag cgaacggcag cggaccacct ttggtaactt tcagtttagc
      721 ggtctgggta ccttcgtacg gacgaccttc accttcacct tcgatttcga actcgtgacc
      781 gttaacggaa ccttccatac gaactttgaa acgcatgaac tctttgataa catcctcgga
      841 ggaagccatc attagaa
//
