LOCUS       sequence-114944-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutively expresses RBPMS in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pFRT-DestFLAGHA_RBPMS
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      123..132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             132..155
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             156..182
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    198..222
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct ggctagcgag ctcggatcga tccatcgata gtgtggtgga attctgatcg
      121 ccgccgccat ggactacaag gacgacgatg acaagtaccc ttatgacgtg cccgattacg
      181 ctttgaacca gatatcaaca agtttgtaca aaaaagcagg ctccaccatg ggaaccaatt
      241 cagtcgacat gaacaacggc ggcaaagccg agaaggagaa caccccgagc gaggccaacc
      301 ttcaggagga ggaggtccgg accctatttg tcagtggcct tcctctggat atcaaacctc
      361 gggagctcta tctgcttttc agaccattta agggctatga gggttctctt ataaagctca
      421 catctaaaca gcctgtaggt tttgtcagtt ttgacagtcg ctcagaagca gaggctgcaa
      481 agaatgcttt gaatggcatc cgcttcgatc ctgaaattcc gcaaacacta cgactagagt
      541 ttgctaaggc aaacacgaag atggccaaga acaaactcgt agggactcca aaccccagta
      601 ctcctctgcc caacactgta cctcagttca ttgccagaga gccatatgag ctcacagtgc
      661 ctgcacttta ccccagtagc cctgaagtgt gggccccgta ccctctgtac ccagcggagt
      721 tagcgcctgc tctacctcct cctgctttca cctatcccgc ttcactgcat gcccagatgc
//
