LOCUS       sequence-114947-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet28a_RBPMS_20_196
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..85
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             89..121
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             704..721
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttgtttact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag
       61 cagcggcctg gtgccgcgcg gcagccatat ggctagcatg actggtggac agcaaatggg
      121 tcgcggatcc gaattcgagc tccgtcgacc catggaggag gaggtccgga ccctatttgt
      181 cagtggcctt cctctggata tcaaacctcg ggagctctat ctgcttttca gaccatttaa
      241 gggctatgag ggttctctta taaagctcac atctaaacag cctgtaggtt ttgtcagttt
      301 tgacagtcgc tcagaagcag aggctgcaaa gaatgctttg aatggcatcc gcttcgatcc
      361 tgaaattccg caaacactac gactagagtt tgctaaggca aacacgaaga tggccaagaa
      421 caaactcgta gggactccaa accccagtac tcctctgccc aacactgtac ctcagttcat
      481 tgccagagag ccatatgagc tcacagtgcc tgcactttac cccagtagcc ctgaagtgtg
      541 ggccccgtac cctctgtacc cagcggagtt agcgcctgct ctacctcctc ctgctttcac
      601 ctatcccgct tcactgcatg cccagatgcg ctggctccct ccctccgagg ctacttctca
      661 gggctggaag tcccgtcagt tctgctgagc ggccgcactc gagcaccacc accaccacca
      721 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
//
