LOCUS       sequence-114949-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-HA expression vector with RBPMS carrying mutation to introduce 
            HindIII site and encoding silent mutation to render resistant to 
            siRNA R2 (Farazi et al. 2014).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_KDR_HindIII
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     45..69
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             169..192
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             193..219
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    235..259
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcagtgaata gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac
       61 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
      121 cgatccagcc tccggactct agcgtttaaa cttaagcttg gtaccatgga ctacaaggac
      181 gacgatgaca agtaccctta tgacgtgccc gattacgctt tgaaccagaa gcttacaagt
      241 ttgtacaaaa aagcaggctc caccatggga accaattcag tcgacatgaa caacggcggc
      301 aaagccgaga aggagaacac cccgagcgag gccaaccttc aggaggagga ggtccggacc
      361 ctatttgtca gtggccttcc tctggacatt aaacctcggg agctctatct gcttttcaga
      421 ccatttaagg gctatgaggg ttctcttata aagctcacat ctaaacagcc tgtaggtttt
      481 gtcagttttg acagtcgctc agaagcagag gctgcaaaga atgctttgaa tggcatccgc
      541 ttcgatcctg aaattccgca aacactacga ctagagtttg ctaaggcaaa cacgaagatg
      601 gccaagaaca aactcgtagg gactccaaac cccagtactc ctctgcccaa cactgtacct
      661 cagttcattg ccagagagcc atatgagctc acagtgcctg cactttaccc cagtagccct
      721 gaagtgtggg ccccgtaccc tctgtaccca gcggagttag cgcctgctct acctcctcct
      781 gctttcacct atcccgcttc actgcatgcc cagatgcgct ggctccctcc ctccgaggct
      841 acttctcagg gctggaagtc ccgtcagttc tgct
//
