LOCUS       sequence-11495-e         408 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Chivers CE, Crozat E, Chu C, Moy VT, Sherratt DJ, Howarth M
  TITLE     A streptavidin variant with slower biotin dissociation and increased
            mechanostability.
  JOURNAL   Nat Methods. 2010 May;7(5):391-3. doi: 10.1038/nmeth.1450. Epub 2010
            Apr 11.
  PUBMED    20383133
REFERENCE   2  (bases 1 to 408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1450"; journalName: "Nat Methods"; date: "2010-05"; 
            volume: "7"; issue: "5"; pages: "391-3"
COMMENT     Sequence Label: pET21a-Traptavidin
FEATURES             Location/Qualifiers
     source          1..408
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             385..402
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggctgaag ctggtatcac cggcacctgg tacaaccagc tgggatccac cttcatcgtt
       61 accgctggtg ctgacggtgc tctgaccggt acctacgaat ccgctgttgg taacgctgaa
      121 ggcgattacg ttctgaccgg tcgttacgac tccgctccgg ctaccgacgg ttccggaacc
      181 gctctgggtt ggaccgttgc ttggaaaaac aactaccgta acgctcactc cgctaccacc
      241 tggtctggcc agtacgttgg tggtgctgaa gctcgtatca acacccagtg gttgttgacc
      301 tccggcacca ccgaagctaa cgcgtggaaa tccaccctgg ttggtcacga caccttcacc
      361 aaagttaaac cgtccgctgc ttcccatcac catcaccacc attaataa
//
