LOCUS       sequence-114950-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-HA expression vector with RBPMS carrying mutation to introduce 
            HindIII site and encoding silent mutation to render resistant to 
            siRNA R2 (Farazi et al. 2014).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_KDR_HindIII
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..34
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     44..68
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             168..191
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             192..218
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    234..258
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc
       61 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
      121 gatccagcct ccggactcta gcgtttaaac ttaagcttgg taccatggac tacaaggacg
      181 acgatgacaa gtacccttat gacgtgcccg attacgcttt gaaccagaag cttacaagtt
      241 tgtacaaaaa agcaggctcc accatgggaa ccaattcagt cgacatgaac aacggcggca
      301 aagccgagaa ggagaacacc ccgagcgagg ccaaccttca ggaggaggag gtccggaccc
      361 tatttgtcag tggccttcct ctggacatta aacctcggga gctctatctg cttttcagac
      421 catttaaggg ctatgagggt tctcttataa agctcacatc taaacagcct gtaggttttg
      481 tcagttttga cagtcgctca gaagcagagg ctgcaaagaa tgctttgaat ggcatccgct
      541 tcgatcctga aattccgcaa acactacgac tagagtttgc taaggcaaac acgaagatgg
      601 ccaagaacaa actcgtaggg actccaaacc ccagtactcc tctgcccaac actgtacctc
      661 agttcattgc cagagagcca tatgagctca cagtgcctgc actttacccc agtagccctg
      721 aagtgtgggc cccgtaccct ctgtacccag cggagttagc gcctgctcta cctcctcctg
      781 ctttcaccta tcccgcttca ctgcatgccc agatgcgctg gctccctccc tccgaggcta
      841 cttctcaggg ctgggaagtc ccgtcagttc tgct
//
