LOCUS       sequence-114951-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-HA expression vector with RBPMS carrying mutation to introduce 
            HindIII site and encoding silent mutation to render resistant to 
            siRNA R2 (Farazi et al. 2014).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_KDR_HindIII
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..75)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    128..152
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atcagttatc tatgcggccg cggatcaggt acgcgtagaa tcgagaccga ggagagggtt
       61 agggataggc ttaccttcga accgcgggcc ctctagactt gagcggccgc cactgtgctg
      121 gatatcaacc actttgtaca agaaagctgg gtctagatat ctcgagtgcg gccgctcagc
      181 agaactgacg ggacttccag ccctgagaag tagcctcgga gggagggagc cagcgcatct
      241 gggcatgcag tgaagcggga taggtgaaag caggaggagg tagagcaggc gctaactccg
      301 ctgggtacag agggtacggg gcccacactt cagggctact ggggtaaagt gcaggcactg
      361 tgagctcata tggctctctg gcaatgaact gaggtacagt gttgggcaga ggagtactgg
      421 ggtttggagt ccctacgagt ttgttcttgg ccatcttcgt gtttgcctta gcaaactcta
      481 gtcgtagtgt ttgcggaatt tcaggatcga agcggatgcc attcaaagca ttctttgcag
      541 cctctgcttc tgagcgactg tcaaaactga caaaacctac aggctgttta gatgtgagct
      601 ttataagaga accctcatag cccttaaatg gtctgaaaag cagatagagc tcccgaggtt
      661 taatgtccag aggaaggcca ctgacaaata gggtccggac ctcctcctcc tgaaggttgg
      721 cctcgctcgg ggtgttctcc ttctcggctt tgccgccgtt gttcatgtcg
//
