LOCUS       sequence-114953-         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synchronize trafficking of ManII from the ER (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: Ii-Str_ManII-SBP-EGFP
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             527..637
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     primer_bind     complement(689..710)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
       61 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
      121 gataagcttg ccacaacccg ggaggcgcgc catgaagtta agtcgccagt tcaccgtgtt
      181 tggcagcgcg atcttctgcg tcgtaatctt ctcactctac ctgatgctgg acaggggtca
      241 cttggactac cctcggggcc cgcgccagga gggctccttt ccgcagggcc agctttcaat
      301 attgcaagaa aagattgacc atttggagcg tttgctcgct gagaacaacg agattatctc
      361 aaatatcaga gactcagtca tcaacctgag cgagtctgtg gaggacggcc cgcgggggtc
      421 accaggcaac gccagccaag gctccatcca cctccactcg ccacagttgg ccctgcaggc
      481 tgaccccaga gactgtttgg atcccaccgg tcgccaccgg aattccgacg agaagaccac
      541 tggttggcga ggtggacacg ttgttgaagg actggctggg gaacttgaac aacttcgtgc
      601 acgactggag catcacccac aaggtcaacg tgaaccacct gcaggtgtga gcaagggcga
      661 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      721 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      781 gttcatctgc accaccggca agctg
//
