LOCUS       sequence-114955-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-HA expression vector with RBPMS carrying deletion at amino acid
            111 (1-111) and encoding silent mutation to render resistant to 
            siRNA R2 (Farazi et al. 2014).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_KDR_111del
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..68)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    121..145
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(786..810)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(826..852)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(853..867)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 atctatgcgg ccgcggatca ggtacgcgta gaatcgagac cgaggagagg gttagggata
       61 ggcttacctt cgaaccgcgg gccctctaga cttgagcggc cgccactgtg ctggatatca
      121 accactttgt acaagaaagc tgggtctaga tatctcgagt gcggccgctc agcagaactg
      181 acgggacttc cagccctgag aagtagcctc ggagggaggg agccagcgca tctgggcatg
      241 cagtgaagcg ggataggtga aagcaggagg aggtagagca ggcgctaact ccgctgggta
      301 cagagggtac ggggcccaca cttcagggct actggggtaa agtgcaggca ctgtgagctc
      361 atatggctct ctggcaatga actgaggtac agtgttgggc agaggagtac tggggtttgg
      421 agttcatacg agtttgttct tggccatctt cgtgtttgcc ttagcaaact ctagtcgtag
      481 tgtttgcgga atttcaggat cgaagcggat gccattcaaa gcattctttg cagcctctgc
      541 ttctgagcga ctgtcaaaac tgacaaaacc tacaggctgt ttagatgtga gctttataag
      601 agaaccctca tagcccttaa atggtctgaa aagcagatag agctcccgag gtttaatgtc
      661 cagaggaagg ccactgacaa atagggtccg gacctcctcc tcctgaaggt tggcctcgct
      721 cggggtgttc tccttctcgg ctttgccgcc gttgttcatg tcgactgaat tggttcccat
      781 ggtggagcct gcttttttgt acaaacttgt tgatatctgg ttcaaagcgt aatcgggcac
      841 gtcataaggg tacttgtcat cgtcgtcctt gtagt
//
