LOCUS       sequence-114958-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-HA expression vector with RBPMS carrying mutation K100A and 
            encoding silent mutation to render resistant to siRNA R2 (Farazi et 
            al. 2014).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_KDR_K100A
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     46..70
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             170..193
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             194..220
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    236..260
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
       61 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      121 ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccatgg actacaagga
      181 cgacgatgac aagtaccctt atgacgtgcc cgattacgct ttgaaccaga tatcaacaag
      241 tttgtacaaa aaagcaggct ccaccatggg aaccaattca gtcgacatga acaacggcgg
      301 caaagccgag aaggagaaca ccccgagcga ggccaacctt caggaggagg aggtccggac
      361 cctatttgtc agtggccttc ctctggacat taaacctcgg gagctctatc tgcttttcag
      421 accatttaag ggctatgagg gttctcttat aaagctcaca tctaaacagc ctgtaggttt
      481 tgtcagtttt gacagtcgct cagaagcaga ggctgcaaag aatgctttga atggcatccg
      541 cttcgatcct gaaattccgc aaacactacg actagagttt gctgcggcaa acacgaagat
      601 ggccaagaac aaactcgtag ggactccaaa ccccagtact cctctgccca acactgtacc
      661 tcagttcatt gccagagagc catatgagct cacagtgcct gcactttacc ccagtagccc
      721 tgaagtgtgg gccccgtacc ctctgtaccc agcggagtta gcgcctgctc tacctcctcc
      781 tgctttcacc tatcccgctt cactgcatgc ccagatgcgc tggctccct
//
