LOCUS       sequence-114961-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of ABKAR biosensor targeted to the lysozome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: LAMP1-ABKAR
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     440..461
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(567..588)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tactactagg agaagacggt aaccttttat tgaatgacat ttccactaat gggacctggt
       61 taaatgggca aaaagtcgag aagaacagca atcagttact gtctcaaggt gatgaaataa
      121 ccgttggtgt aggcgtggaa tcagatattt tatctctggt cattttcata aacgacaaat
      181 ttaagcagtg cctcgagcag aacaaagttg atcgctctgc aggtaagcca ggcagcggcg
      241 agggcagcac caagatgcgt cgtgtcgcca ccctggtgga cctcggcacc ggcggcagcg
      301 agctcatggg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      361 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaagctg agcaaagacc
      421 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      481 tcggcatgga cgagctgtac aagggaggta ccggtggatc tatggtgagc aagggcgagg
      541 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      601 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc
      661 tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct
      721 acggccttca gtgcttcg
//
