LOCUS       sequence-114962-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pRET2 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK020
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(832..866)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
       61 tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg tctcaaacga cgatggcttc
      121 ttatctcact tcaatagtac tttccaccgg ttatacttcc ggcttttccc tattaataca
      181 agctacaatt tcaatgggtg gcaaataatg tgtagaatag aaaataagcc gacagggtaa
      241 taaagaaaat ttttagaaaa aaaaggttag atggcttatt taagttacag gctagcgaaa
      301 aaaggaactt cagggcaagt aaagtgtttg attgggcact agcatggctt ataaaggcga
      361 gcaattgtcg aaactaatta atgttgtacg gactattgct gtcatctcgt ggtaaatgcg
      421 tgttccaggt cgaatactac ttgcacacag gcgagcgggg ccccataaaa gtgttgccga
      481 tttgttaagt tgtcttttcg gtttttctac tctgttattc cttacttccc tttttaagaa
      541 ctctttttat ccttcattta ggatcttgca cgtttccgcc tcatcacttg aattaaaaca
      601 tgtctctgtc agtaaacctt ggcgtttcta ttgttcttca tagttcaact tttattatta
      661 cccgccctgc gcgtttacat ttttccagca acagccagcg aaaaattaga aaatctggtt
      721 gttgacacct caagaacaag ggcaattagc ctcagcgtcg aatatagatc atattagaat
      781 acctatagct ccatcaaaag aaatacacaa gatctatgtg agaccagacc aataaaaaac
      841 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
      901 tctatcaaca ggagtccaag cgagctcgat at
//
