LOCUS       sequence-114968-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP 
            expression cassette - Kanamycin plasmid 
            [E:J23102:B:BCD2:C:E0040m:D:B0015:F].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: pJ02B2Gm_EF
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..79)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    80..100
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(119..146)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(162..233)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(296..316)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 agcctgcata acgcgaagta atcttttcgg ttttaaagaa aaagggcagg gtggtgacac
       61 cttgcccttt tttgccggac tgcagcggcc gctactagta gaagacatag cgtataaacg
      121 cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt caccgacaaa
      181 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg
      241 gacctacctt tattatttgt atagttcatc catgccatgt gtaatcccag cagctgttac
      301 aaactcaaga aggaccatgt gatctctctt ttcgttggga tctttcgaaa gggcagattg
      361 tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg
      421 ttgataatgg tctgctagtt gaacgcttcc atcttcaatg ttgtgtctaa ttttgaagtt
      481 aactttgatt ccattctttt gtttgtctgc catgatgtat acattgtgtg agttatagtt
      541 gtattccaat ttgtgtccaa gaatgtttcc atcttcttta aaatcaatac cttttaactc
      601 gattctatta acaagggtat caccttcaaa cttgacttca gcacgtgtct tgtagttccc
      661 gtcatctttg aaaaatatag ttctttcctg tacataacct tcgggcatgg cactcttgaa
      721 aaagtcatgc tgtttcatat gatctgggta tctcgcaaag cattgaacac cataaccgaa
      781 agtagtgaca agtgttggcc atggaacagg tagtt
//
