LOCUS       sequence-114970-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP 
            expression cassette - Kanamycin plasmid 
            [A:J23102:B:BCD2:C:E1010m:D:B0015:E].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: pJ02B2Rm_AE
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..79)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    80..100
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(119..146)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(162..233)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agcctgcata acgcgaagta atcttttcgg ttttaaagaa aaagggcagg gtggtgacac
       61 cttgcccttt tttgccggac tgcagcggcc gctactagta gaagacataa gctataaacg
      121 cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt caccgacaaa
      181 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg
      241 gacctttatt aagcaccggt ggagtgacga ccttcagcac gttcgtactg ttcaacgatg
      301 gtgtagtcct cgttgtggga ggtgatgtcc agtttgatgt cggttttgta agcacccggc
      361 agctgaaccg gttttttagc catgtaggtg gttttaactt cagcgtcgta gtgaccaccg
      421 tctttcagtt tcagacgcat tttgatttca cctttcagag caccatcctc cgggtacata
      481 cgttcggtgg aagcttccca acccatggtt tttttctgca taaccggacc gtcggacggg
      541 aagttggtac cacgcagttt aactttgtag atgaactcac cgtcttgcag ggaggagtcc
      601 tgggtaacgg taacaacacc accatcttcg aagttcataa cacgttccca tttgaaacct
      661 tccgggaagg acagtttcag gtagtccggg atgtcagccg ggtgtttaac gtaagctttg
      721 gaaccgtact ggaactgcgg ggacaggatg tcccaagcga acggcagcgg accacctttg
      781 gtaactttca gtttagcggt ctgggtacct tcgtacggac gaccttcacc ttcaccttcg
      841 att
//
