LOCUS       sequence-114973-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP 
            expression cassette - Kanamycin plasmid 
            [E:J23102:B:BCD2:C:E1010m:D:B0015:F].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: pJ02B2Rm_EF
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(21..78)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    79..99
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(118..145)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(161..232)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcctgcataa cgcgaagtaa tcttttcggt tttaaagaaa aagggcaggg tggtgacacc
       61 ttgccctttt ttgccggact gcagcggccg ctactagtag aagacatagc gtataaacgc
      121 agaaaggccc acccgaaggt gagccagtgt gactctagta gagagcgttc accgacaaac
      181 aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg
      241 acctttatta agcaccggtg gagtgacgac cttcagcacg ttcgtactgt tcaacgatgg
      301 tgtagtcctc gttgtgggag gtgatgtcca gtttgatgtc ggttttgtaa gcacccggca
      361 gctgaaccgg ttttttagcc atgtaggtgg ttttaacttc agcgtcgtag tgaccaccgt
      421 ctttcagttt cagacgcatt ttgatttcac ctttcagagc accatcctcc gggtacatac
      481 gttcggtgga agcttcccaa cccatggttt ttttctgcat aaccggaccg tcggacggga
      541 agttggtacc acgcagttta actttgtaga tgaactcacc gtcttgcagg gaggagtcct
      601 gggtaacggt aacaacacca ccatcttcga agttcataac acgttcccat ttgaaacctt
      661 ccgggaagga cagtttcagg tagtccggga tgtcagccgg gtgtttaacg taagctttgg
      721 aaccgtactg gaactgcggg gacaggatgt cccaagcgaa cggcagcgga ccacctttgg
      781 taactttcag tttagcggtc tgggtacctt cgtacggacg accttcacct tcaccttcga
      841 tttc
//
