LOCUS       sequence-114981-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mTagBFP-GFP2-FRET
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..762
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 accatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
      121 gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
      181 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
      241 actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc
      301 ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
      361 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
      421 aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc
      481 tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac
      541 atggccctga agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga
      601 tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg
      661 gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc
      721 agatactgcg acctccctag caaactgggg cacaagctta attccggact cagatcccca
      781 ccggtcgcca ccatgagcgg gggcgaggag ctgttcgccg gcatcgtgcc cgtgctgatc
      841 gagctggacg gcgacgtgca cggccacaag ttca
//
