LOCUS       sequence-114982-        3379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry vector with HuD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3379)
  TITLE     Identification of the transcriptome-wide mRNA targets of the 
            neuronal ELAVL proteins (unpublished)
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENTRY4_HuD
FEATURES             Location/Qualifiers
     source          1..3379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1608..1707)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1819..1838)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1830..2639
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1897..1916)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2729..3317
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3218..3237
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatgggaac caattcagtc
      481 gacatggtta tgataattag caccatggag cctcaggtgt caaatggtcc gacatccaat
      541 acaagcaatg gaccctccag caacaacaga aactgtcctt ctcccatgca aacaggggca
      601 accacagatg acagcaaaac caacctcatc gtcaactatt taccccagaa tatgacccaa
      661 gaagaattca ggagtctctt cgggagcatt ggtgaaatag aatcctgcaa acttgtgaga
      721 gacaaaatta caggacagag tttagggtat ggatttgtta actatattga tccaaaggat
      781 gcagagaaag ccatcaacac tttaaatgga ctcagactcc agaccaaaac cataaaggtc
      841 tcatatgccc gtccgagctc tgcctcaatc agggatgcta acctctatgt tagcggcctt
      901 cccaaaacca tgacccagaa ggaactggag caacttttct cgcaatacgg ccgtatcatc
      961 acctcacgaa tcctggttga tcaagtcaca ggagtgtcca gaggggtggg attcatccgc
     1021 tttgataaga ggattgaggc agaagaagcc atcaaagggc tgaatggcca gaagcccagc
     1081 ggtgctacgg aaccgattac tgtgaagttt gccaacaacc ccagccagaa gtccagccag
     1141 gccctgctct cccagctcta ccagtcccct aaccggcgct acccaggtcc acttcaccac
     1201 caggctcaga ggttcaggct ggacaatttg cttaatatgg cctatggcgt aaagaggttc
     1261 tccccaatta ccattgatgg aatgacaagc cttgtgggaa tgaacatccc tggtcacaca
     1321 ggaactgggt ggtgcatctt tgtctacaac ctgtcccccg attccgatga gagtgtcctc
     1381 tggcagctct ttggcccctt tggagcagtg aacaacgtaa aggtgattcg tgacttcaac
     1441 accaacaagt gcaagggatt cggctttgtc accatgacca actatgatga ggcggccatg
     1501 gccatcgcca gcctcaacgg gtaccgcctg ggagacagag tgttgcaagt ttcctttaaa
     1561 accaacaaag cccacaagtc ctgagcggcc gcactcgaga tatctagacc cagctttctt
     1621 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     1681 tatcagtcaa aataaaatca ttatttgcca tccagctgca gctctggccc gtgtctcaaa
     1741 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
     1801 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
     1861 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     1921 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
     1981 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     2041 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctggtg
     2101 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
     2161 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     2221 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     2281 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     2341 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     2401 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     2461 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     2521 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     2581 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     2641 cagaattggt taattggttg taacattatt cagattgggc cccgttccac tgagcgtcag
     2701 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2761 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2821 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2881 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2941 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3001 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3061 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3121 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3181 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3241 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3301 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3361 ggccttttgc tcacatgtt
//
