LOCUS       sequence-114985-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mTagBFP-GFP2-FRET
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(126..842)
                     /codon_start=1
                     /product="monomeric green fluorescent protein, also known 
                     as mTagGFP"
                     /label=TagGFP2
                     /note="mammalian codon-optimized"
                     /translation="MSGGEELFAGIVPVLIELDGDVHGHKFSVRGEGEGDADYGKLEIK
                     FICTTGKLPVPWPTLVTTLCYGIQCFARYPEHMKMNDFFKSAMPEGYIQERTIQFQDDG
                     KYKTRGEVKFEGDTLVNRIELKGKDFKEDGNILGHKLEYSFNSHNVYIRPDKANNGLEA
                     NFKTRHNIEGGGVQLADHYQTNVPLGDGPVLIPINHYLSTQTKISKDRNEARDHMVLLE
                     SFSACCHTHGMDELYR"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg
      121 gccgcttacc tgtacagctc gtccatgccg tgggtgtggc agcaggcgct gaaggactcc
      181 aggagcacca tgtggtcgcg ggcctcgttg cggtccttgc tgatcttggt ctgagtgctc
      241 aggtagtggt tgatggggat cagcacgggg ccgtcgccca ggggcacgtt ggtctggtag
      301 tggtcggcca gctgcacgcc gccgccctcg atgttgtggc gggtcttgaa gttagcctcc
      361 aggccgttgt tggccttgtc ggggcggatg tacacgttgt ggctgttgaa gctgtactcc
      421 agcttgtggc ccaggatgtt gccgtcctcc ttgaagtcct tgcccttcag ctcgatgcgg
      481 ttcaccaggg tgtcgccctc gaacttcacc tcgccgcggg tcttgtactt gccgtcgtcc
      541 tggaactgga tggtgcgctc ctggatgtag ccctcgggca tggcgctctt gaagaagtcg
      601 ttcatcttca tgtgctcggg gtagcgggcg aagcactgga tgccgtagca gagggtggtc
      661 accagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt gatctccagc
      721 ttgccgtagt cggcgtcgcc ctcgccctcg ccgcgcacgc tgaacttgtg gccgtgcacg
      781 tcgccgtcca gctcgatcag cacgggcacg atgccggcga acagctcctc gcccccgctc
      841 atggtggcga ccggtgggga tctga
//
