LOCUS       sequence-114987-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses FLAGHA-tagged HuB in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  TITLE     Identification of the transcriptome-wide mRNA targets of the 
            neuronal ELAVL proteins (unpublished)
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFRT-TODestFLAGHA_HuB
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     48..72
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             172..195
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             196..222
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    238..262
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
       61 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      121 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccat ggactacaag
      181 gacgacgatg acaagtaccc ttatgacgtg cccgattacg ctttgaacca gatatcaaca
      241 agtttgtaca aaaaagcagg ctccaccatg ggaaccaatt cagtcgactg gatccggatg
      301 gaaacacaac tgtctaatgg gccaacttgc aataacacag ccaatggtcc aaccaccata
      361 aacaacaact gttcgtcacc agttgactct gggaacacag aagacagcaa gaccaactta
      421 atagtcaact accttcctca gaacatgaca caggaggaac taaagagtct ctttgggagc
      481 attggtgaaa tagagtcctg taagcttgta agagacaaaa taacagggca gagcttggga
      541 tatggctttg tgaactacat tgaccccaag gatgcagaga aagctatcaa caccctgaat
      601 ggattgagac ttcaaaccaa aacaataaaa gtttcctatg ctcgcccaag ttcagcttct
      661 atcagagatg caaatttata tgtcagcgga cttccaaaaa caatgaccca gaaggagttg
      721 gaacagcttt tttcacaata tggacgcatt attacttctc gtattcttgt cgaccaggtc
//
