LOCUS       sequence-114988-        1634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mTagBFP-GFP2-FRET
FEATURES             Location/Qualifiers
     source          1..1634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..762
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             793..1509
                     /codon_start=1
                     /product="monomeric green fluorescent protein, also known 
                     as mTagGFP"
                     /label=TagGFP2
                     /note="mammalian codon-optimized"
                     /translation="MSGGEELFAGIVPVLIELDGDVHGHKFSVRGEGEGDADYGKLEIK
                     FICTTGKLPVPWPTLVTTLCYGIQCFARYPEHMKMNDFFKSAMPEGYIQERTIQFQDDG
                     KYKTRGEVKFEGDTLVNRIELKGKDFKEDGNILGHKLEYSFNSHNVYIRPDKANNGLEA
                     NFKTRHNIEGGGVQLADHYQTNVPLGDGPVLIPINHYLSTQTKISKDRNEARDHMVLLE
                     SFSACCHTHGMDELYR"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 accatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
      121 gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
      181 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
      241 actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc
      301 ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
      361 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
      421 aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc
      481 tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac
      541 atggccctga agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga
      601 tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg
      661 gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc
      721 agatactgcg acctccctag caaactgggg cacaagctta attccggact cagatcccca
      781 ccggtcgcca ccatgagcgg gggcgaggag ctgttcgccg gcatcgtgcc cgtgctgatc
      841 gagctggacg gcgacgtgca cggccacaag ttcagcgtgc gcggcgaggg cgagggcgac
      901 gccgactacg gcaagctgga gatcaagttc atctgcacca ccggcaagct gcccgtgccc
      961 tggcccaccc tggtgaccac cctctgctac ggcatccagt gcttcgcccg ctaccccgag
     1021 cacatgaaga tgaacgactt cttcaagagc gccatgcccg agggctacat ccaggagcgc
     1081 accatccagt tccaggacga cggcaagtac aagacccgcg gcgaggtgaa gttcgagggc
     1141 gacaccctgg tgaaccgcat cgagctgaag ggcaaggact tcaaggagga cggcaacatc
     1201 ctgggccaca agctggagta cagcttcaac agccacaacg tgtacatccg ccccgacaag
     1261 gccaacaacg gcctggaggc taacttcaag acccgccaca acatcgaggg cggcggcgtg
     1321 cagctggccg accactacca gaccaacgtg cccctgggcg acggccccgt gctgatcccc
     1381 atcaaccact acctgagcac tcagaccaag atcagcaagg accgcaacga ggcccgcgac
     1441 cacatggtgc tcctggagtc cttcagcgcc tgctgccaca cccacggcat ggacgagctg
     1501 tacaggtaag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
     1561 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     1621 tgttgttgtt aact
//
