LOCUS       sequence-114990-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses FLAGHA-tagged HuB in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  TITLE     Identification of the transcriptome-wide mRNA targets of the 
            neuronal ELAVL proteins (unpublished)
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFRT-TODestFLAGHA_HuB
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..74)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    127..151
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcagttatct atgcggccgc ggatcaggta cgcgtagaat cgagaccgag gagagggtta
       61 gggataggct taccttcgaa ccgcgggccc tctagacttg agcggccgcc actgtgctgg
      121 atatcaacca ctttgtacaa gaaagctggg tctagatatc tcgagtgcgg ccgctcatta
      181 ggctttgtgc gttttgtttg tcttaaagga gacctgcagt actctgtctc ccagacggta
      241 tccattgagg ctagctatcg ccatggcagc ctcatcatag tttgtcatag tcacaaatcc
      301 aaaacctttg catttattgg tgttaaagtc acggatgacc ttcacattgg tgacagctcc
      361 aaaaggccca aacatttgcc acaggatact ctcatctgcg tcaggagcca ggttgtacac
      421 aaatatacac caccctgttc cagggtgccc agggatatta attccagcca aactggtcat
      481 tccgtcaatg gtcattggag aaaacctctt tactccataa gccatattga gcagattgtc
      541 caacctaaaa cgctgtgcct gctgagctag cggtcctgga taccttctgt ttggagactg
      601 gtacagctgg gaaaggatgg cctgattggt tttttggctt gggttattag caaactttac
      661 agtgattggc tccgtggcac cgggaggttt ctggccattt aggcctttga tagcttcttc
//
