LOCUS       sequence-114992-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses FLAGHA-tagged HuC in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  TITLE     Identification of the transcriptome-wide mRNA targets of the 
            neuronal ELAVL proteins (unpublished)
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFRT-TODestFLAGHA_HuC
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             163..186
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             187..213
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    229..253
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgttttagt gaaccgtcag
       61 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      121 agcctccgga ctctagcgtt taaacttaag cttggtacca tggactacaa ggacgacgat
      181 gacaagtacc cttatgacgt gcccgattac gctttgaacc agatatcaac aagtttgtac
      241 aaaaaagcag gctccaccat gggaaccaat tcagtcgaca tggtcactca gatactgggg
      301 gccatggagt ctcaggtggg ggggggcccg gccggcccgg ccctgcccaa cgggccactc
      361 cttggtacaa atggagccac tgacgacagc aagaccaacc tcatcgtcaa ctacctgccc
      421 cagaacatga cccaggatga gttcaagagt ctcttcggca gcattggcga catcgagtcc
      481 tgcaagttgg ttcgggacaa gatcacaggg cagagccttg gctacgggtt tgtgaactat
      541 tctgacccca atgatgcaga caaagccatc aacaccctca acggcctcaa attacagacg
      601 aagaccatca aggtgtccta tgccagaccc agttcagcat ccatccggga tgctaacctg
      661 tacgtcagcg ggctccccaa gaccatgagc cagaaagaga tggagcagct cttctcccag
//
