LOCUS       sequence-114994-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses FLAGHA-tagged HuC in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  TITLE     Identification of the transcriptome-wide mRNA targets of the 
            neuronal ELAVL proteins (unpublished)
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFRT-TODestFLAGHA_HuC
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     38..62
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             162..185
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             186..212
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    228..252
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga
       61 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      121 gcctccggac tctagcgttt aaacttaagc ttggtaccat ggactacaag gacgacgatg
      181 acaagtaccc ttatgacgtg cccgattacg ctttgaacca gatatcaaca agtttgtaca
      241 aaaaagcagg ctccaccatg ggaaccaatt cagtcgacat ggtcactcag atactggggg
      301 ccatggagtc tcaggtgggg gggggcccgg ccggcccggc cctgcccaac gggccactcc
      361 ttggtacaaa tggagccact gacgacagca agaccaacct catcgtcaac tacctgcccc
      421 agaacatgac ccaggatgag ttcaagagtc tcttcggcag cattggcgac atcgagtcct
      481 gcaagttggt tcgggacaag atcacagggc agagccttgg ctacgggttt gtgaactatt
      541 ctgaccccaa tgatgcagac aaagccatca acaccctcaa cggcctcaaa ttacagacga
      601 agaccatcaa ggtgtcctat gccagaccca gttcagcatc catccgggat gctaacctgt
      661 acgtcagcgg gctccccaag accatgagcc agaaagagat ggagcagctc ttctcccag
//
