LOCUS       sequence-114998-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutively expresses FLAG-HA-tagged HuB in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  TITLE     Identification of the transcriptome-wide mRNA targets of the 
            neuronal ELAVL proteins (unpublished)
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFRT-DestFLAGHA_HuB
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        31..49
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      114..123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             123..146
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             147..173
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    189..213
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
       61 tggctagcga gctcggatcg atccatcgat agtgtggtgg aattctgatc gccgccgcca
      121 tggactacaa ggacgacgat gacaagtacc cttatgacgt gcccgattac gctttgaacc
      181 agatatcaac aagtttgtac aaaaaagcag gctccaccat gggaaccaat tcagtcgact
      241 ggatccggat ggaaacacaa ctgtctaatg ggccaacttg caataacaca gccaatggtc
      301 caaccaccat aaacaacaac tgttcgtcac cagttgactc tgggaacaca gaagacagca
      361 agaccaactt aatagtcaac taccttcctc agaacatgac acaggaggaa ctaaagagtc
      421 tctttgggag cattggtgaa atagagtcct gtaagcttgt aagagacaaa ataacagggc
      481 agagcttggg atatggcttt gtgaactaca ttgaccccaa ggatgcagag aaagctatca
      541 acaccctgaa tggattgaga cttcaaacca aaacaataaa agtttcctat gctcgcccaa
      601 gttcagcttc tatcagagat gcaaatttat atgtcagcgg acttccaaaa acaatgaccc
      661 agaaggagtt ggaacagctt ttttcacaat atggacgcat tattacttct cgtattcttg
      721 tcgaccaggt cactggcata tcaaggggtg tagggtttat tcgatttgac
//
